Differential Expression of Procollagen Lysine 2-\ud Oxoglutarate 5-Deoxygenase and Matrix\ud Metalloproteinase Isoforms in Hypothyroid Rat Ovary\ud and Disintegration of Extracellular Matrix

摘要

Hypothyroid-induced reproductive malfunction in both the\udsexes is a common phenomenon of global concern. In an attempt\udto characterize the differentially expressed genes that\udmight be responsible for these disorders, we have identified a\udnumber of clones in hypothyroid rat ovary by subtractive\udhybridization. One such clone is procollagen lysyl hydroxylase2\ud(Plod-2), the key enzyme for the first step of collagen\udbiosynthetic pathway, which was down-regulated in hypothyroid\udcondition. We have also demonstrated the reduced expression\udof other isoforms of Plods, namely Plod-1 and -3 in\udhypothyroid rat ovary. The current studies are the first of their kind to report that thyroid hormone regulates the Plod\udgene in rat ovary. Moreover, we have shown the up-regulation\udof matrix-degrading enzyme(s), matrix metalloproteinase(s)\udin the hypothyroid rat ovary, whereas the tissue-inhibitory\udmetalloproteinase is down-regulated. Finally, the results of\udthe present studies indicate that in hypothyroid condition,\udcollagen biosynthesis in ovary seems to be disturbed with\udconcomitant enhancement in collagen degradation, resulting\udin disintegration of overall ovarian structure. (Endocrinology\ud146: 2963–2975, 2005)